Dr. Keith C.L. LEE 李卓倫博士

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Research Officer

PhD (HK)

location Rm 747, Laboratory Block, LKS faculty of Medicine, The University of Hong Kong
phone (852)-3917-9388
fax (852)-2816-1947
email HKU Scholars Hub
email https://orcid.org/0000-0003-1553-3478
Research Interests
  • Reproductive Immunology
  • Placental Development
  • Glycobiology
  • Lee CL, Chen Z, Zhang Q, Guo Y, Ng VWY, Zhang B, Bai K, Ruan D, Kan ASY, Cheung KW, Mak ASL, Yeung WSB, Su R, Yang Q, Chen M, Du MR, Jian Z, Fan X, Chiu PCN. Dysregulation of the CD147 complex confers defective placental development: A pathogenesis of early-onset preeclampsia. Clin Transl Med. 2022 Jun;12(6):e826. doi: 10.1002/ctm2.826
  • BAI K., Lee C.L., Liu X., LI J., Cao D., Zhang L., Hu D., Li H., Hou Y., Xu Y., Kan S.Y.A., Cheung K.W., Ng E.H.Y., Yeung W.S.B. and Chiu C.N., Human placental exosomes induce maternal systemic immune tolerance by reprogramming circulating monocytes, Journal of Nanobiotechnology. 2022, 20.
  • Liu Z, Zhai M, Zhang Q, Yang T, Wan Z, Li J, Liu X, Xu B, Du L, Chan RWS, Zhang L, Yeung WSB, Cheung KW, Chiu PCN, Wang WJ, Lee CL, Gao Y. Resolving the gene expression maps of human first-trimester chorionic villi with spatial transcriptome. Front Cell Dev Biol. 2022 Dec 6;10:1060298. doi: 10.3389/fcell.2022.1060298.
  • Li X, Kodithuwakku SP, Chan RWS, Yeung WSB, Yao Y, Ng EHY, Chiu PCN, Lee CL. Three-dimensional culture models of human endometrium for studying trophoblast-endometrium interaction during implantation. Reprod Biol Endocrinol. 2022 Aug 13;20(1):120.
  • Cheng Y, Wang H, Shang J, Wang J, Yin J, Zhang J, Guo X, Wang S, Duan YG, Lee CL, Chiu PCN, Zhang J, Yeung WSB, Cao D, Yao Y. Transcriptomic analysis of mid-secretory endometrium reveals essential immune factors associated with pregnancy after single euploid blastocyst transfer. Am J Reprod Immunol. 2022 Dec 21. doi: 10.1111/aji.13672.
  • Fernando S.R., Lee C.L., Wong B.P.C., Cheng K.W., Lee C.Y.L., Chan M.C., Ng E.H.Y., Yeung W.S.B. and Lee C.K.F., Expression of membrane protein disulphide isomerase A1 (PDIA1) disrupt a reducing microenvironment in endometrial epithelium for embryo implantation, Experimental Cell Research. 2021, 405, 112665. http://dx.doi.org/10.1016/j.yexcr.2021.112665
  • Fernando SR, Kottawatta KSA, Jiang L, Chen X, Cheng KW, Wong BP, Ng EH, Yeung WS, Lee KF. Differential expression of protein disulfide isomerase (PDI) in regulating endometrial receptivity in humans. Reprod Biol. 2021 Jun;21(2):100498. doi: 10.1016/j.repbio.2021.100498. Epub 2021 Mar 4. PMID: 33677360.
  • Fernando SR, Lee CL, Wong BP, Cheng KW, Lee YL, Chan MC, Ng EH, Yeung WS, Lee KF. Expression of membrane protein disulphide isomerase A1 (PDIA1) disrupt a reducing microenvironment in endometrial epithelium for embryo implantation. Exp Cell Res. 2021 Jun 8;405(2):112665. doi: 10.1016/j.yexcr.2021.112665. Epub ahead of print. PMID: 34111473.
  • Li Y.T., Zhong L.Y., Lee C.L., Chiu C.N. and Chen M., Identification of Adrenomedullin-Induced S-Nitrosylated Proteins in JEG-3 Placental Cells, Reproductive Sciences. 2021. http://dx.doi.org/10.1007/s43032-021-00663-7
  • WANG Y., Zhao W., MEI S., Chen P.Y., LEUNG T.Y., Lee C.L., Yeung W.S.B., Ou J.P., Liang X.Y. and Chiu C.N., Identification of Sialyl-Lewis(x)-Interacting Protein on Human Spermatozoa, Frontiers in Cell and Developmental Biology. 2021, 9. http://dx.doi.org/10.3389/fcell.2021.700396
  • BAI K., LI X., ZHONG J., Ng E.H.Y., Yeung W.S.B., Lee C.L. and Chiu C.N., Placenta-Derived Exosomes as a Modulator in Maternal Immune Tolerance During Pregnancy, Frontiers in Immunology. 2021, 12. http://dx.doi.org/10.3389/fimmu.2021.671093
  • Leung ETY, Lee CL, Tian X, Lam KKW, Li RHY, Ng EHY, Yeung WSB, Chiu PCN (2021). Simulating nature in sperm selection for assisted reproduction. Nature Review Urology. Online ahead of print.
  • Vijayan M, Lee CL, Wong VHH, Wang X, Bai K, Wu J, Koistinen H, Seppälä M, Lee KF, Yeung WSB, Ng EHY, Chiu PCN. Decidual glycodelin-A polarizes human monocytes into a decidual macrophage-like phenotype through Siglec-7. J Cell Sci. 2020 Jul 23;133(14):jcs244400. doi: 10.1242/jcs.244400. PMID: 32513821.
  • Hautala LC, Pang PC, Antonopoulos A, Pasanen A, Lee CL, Chiu PCN, Yeung WSB, Loukovaara M, Bützow R, Haslam SM, Dell A, Koistinen H. Altered glycosylation of glycodelin in endometrial carcinoma. Laboratory Investigation.
  • Wang X, Lee CL (Co-first author), Vijayan M, Yeung WSB, Ng EHY, Wang X, Li HWR, Zhang Y, Chiu PCN. Adrenomedullin insufficiency alters macrophage activities in pro-inflammatory conditions: a pathophysiologic explanation of tubal ectopic pregnancy. Mucosal Immunology.
  • Cao M, Chan RWS, Cheng FHC, Xua S, Li J, Li T, Pang RTK, Lee CL, Li RHW, Ng EHY, Chiu PCN, Yeung WSB. Myometrial cells stimulate self-renewal of endometrial mesenchymal stem-like cells through WNT5A/β-catenin signaling. Stem cells. 2019. 37(11):1455–1466.
  • Mei S, Chen P, Lee CL, Zhao W, Wang Y, Lam KKW, Ho PC, Yeung WSB, Fang C, Chiu PCN. The role of galectin-3 in spermatozoa-zona pellucida binding and its association with fertilization in vitro. Molecular Human Reproduction. 2019. 25(8):458-470.
  • Lee C.L., Veerbeek J.H.W., Rana T.K., van Rijn B.B., Burton G.J. and Yung H.W., Endoplasmic reticulum stress reduces matrix metalloproteinase-2 activity via eIF2a-ATF4 pathway: Implications for trophoblast invasion in complicated pregnancies., American Journal of Pathology. 2019, 189, 467-478. http://dx.doi.org/10.1016/j.ajpath.2018.10.015

  • Lee C.L., Vijayan M., Wang X., Lam K.W., Koistinen H., Seppala M., Li R.H.W., Ng E.H.Y., Yeung W.S.B. and Chiu C.N., Glycodelin-A stimulates the conversion of human peripheral blood CD16−CD56bright NK cell to a decidual NK cell-like phenotype, Human Reproduction. 2019, 34, 689-701. http://dx.doi.org/10.1093/humrep/dey378

  • Li S., Ip K.M., Tang Y.H., Tang K.S., Tong Y., Zhang J., Hassan A.A., Mak A.S.C., Yung S.S.Y., Chan D.T.M., Ip P.C.P., Lee C.L., Chiu C.N., Lee L.T.O., Lai H.C., Zeng J.Z., Shum H.C. and Wong A.S.T., Sialyl Lewis~^X~~-P-selectin cascade mediates tumor-mesothelial adhesion in ascitic fluid shear flow, Nat Cummun. 2019, 10(1), 2406. http://dx.doi.org/10.1038/S41467-019-10334-6

  • Wang X., Lee C.L., Li R.H.W., Vijayan M., Duan Y., Yeung W.S.B., Zhang Y.Z. and Chiu C.N., Alteration of the immune cell profiles in the pathophysiology of tubal ectopic pregnancy, American Journal of Reproductive Immunology. 2019, 81, e13093. http://dx.doi.org/10.1111/aji.13093

  • Chan RWS, Lee CL, Ng EH, Yeung WSB. Coculture with Macrophages enhanced the Clonogenic and Invasion Activity of Endometriotic Stromal Cells. Cell Proliferation. 2017;50:e12330.

  • Chan R.W.S., Lee C.L., Ng E.H.Y. and Yeung W.S.B., Co-culture with macrophages enhances the clonogenic and invasion activity of endometriotic stromal cells, Cell Proliferation. John Wiley & Sons Ltd, 2017, 50: 1-9.

  • Wong C.W., Lam K.W., Lee C.L., Yeung W.S.B., Zhao W.E., Ho P.C., Oue J.P. and Chiu C.N., The roles of protein disulphide isomerase family A, member 3 (ERp57) and surface thiol/disulphide exchange in human spermatozoa-zona pellucida binding, Human Reproduction. 2017, 32: 733-742.

  • Lee C.L., Lam K.K., VIJAYAN M., Koistinen H., Seppala M., Ng E.H.Y., Yeung W.S.B. and Chiu C.N., The pleiotropic effect of glycodelin-A in early pregnancy, American Journal of Reproductive Immunology. 2016, 75(3): 290-297.

  • Huang VW, Lee CL, Lee YL, Lam KK, Ko JK, Yeung WSB, Ho PC, Chiu PC. Sperm fucosyltransferase-5 mediates spermatozoa-oviductal epithelial cell interaction to protect human spermatozoa from oxidative damage. Molecular Human Reproduction 2015 Jun;21(6):516-26.

  • Lee CL, Guo Y, So KH, Vijayan M, Wong HHV, Yao Y, Lee CKF, Chiu CN and Yeung WSB. Soluble human leukocyte antigen G5 polarizes differentiation of macrophages towards a decidual macrophage-like phenotype. Human Reproduction 2015, 30: 2263-74.

  • Lee YL, Fong SW, Chen AC, Li T, Yue C, Lee CL, Ng EH, Yeung WS, Lee KF.Establishment of a novel human embryonic stem cell-derived trophoblastic spheroid implantation model. Hum Reprod. 2015 Sep 7. pii: dev223. [Epub ahead of print]

  • Lee CL, Li K, Lam KK, Ng EH, Koistinen H, Seppälä M, Yeung WS, Chiu PC.Glycodelin-A treatment reduces the adverse effect of macrophage co-culture on human sperm motility. Mol Reprod Dev. 2014 Jun;81(6):482-3. doi: 10.1002/mrd.22331. Epub 2014 May 9. [Medline]

  • Guo YF, Lee CL, So KH, Gao J, Yeung WSB, Yao YQ, Lee KF. Soluble Human Leukocyte Antigen-G5 Activates Extracellular Signal-Regulated Protein Kinase Signaling and Stimulates Trophoblast Invasion. PLoS One 2013 Oct 1;8(10):e76023. [PubMed]

  • Huang VW, Zhao W, Lee CL, Lee CY, Lam KK, Ko JK, Yeung WS, Ho PC, Chiu PC. Cell membrane proteins from oviductal epithelial cell line protect human spermatozoa from oxidative damage. Fertil Steril. 2013 Apr;99(5):1444-1452. [PubMed]

  • Lee CL, Chiu PC, Hautala L, Salo T, Yeung WS, Stenman UH, Koistinen H. Human chorionic gonadotropin and its free β-subunit stimulate trophoblast invasion independent of LH/hCG receptor. Mol Cell Endocrinol. 2013 Aug 15;375(1-2):43-52. [PubMed]

  • Lee CL, Lam MPY, Lam KKW, Leung CON, Pang RTK, Chu IK, Wan THL, Chai J, Yeung WSB, Chiu PCN. The identification of CD147 (Basigin) as a mediator of trophoblast functions. Hum Reprod 2013 Sep. [ Epub ahead of print] [PubMed]

  • Pang RT, Leung CO, Lee CL, Lam KK, Ye TM, Chiu PC, Yeung WS. MicroRNA-34a is a tumor suppressor in choriocarcinoma via regulation of Delta-like1. BMC Cancer. 2013 Jan 18;13:25. doi: 10.1186/1471-2407-13-25. [PubMed]

  • Wong BS, Lam KK, Lee CL, Wong VH, Lam MP, Chu IK, Yeung WS, Chiu PC. Adrenomedullin enhances invasion of human extravillous cytotrophoblast-derived cell lines by regulation of urokinase plasminogen activator expression and s-nitrosylation. Biol Reprod. 2013 Feb 14;88(2):34. [PubMed]

  • Chiu CN, Lam KK, Lee CL, Huang VW, Wong BS, Zhao W, Li K, Ho PC, Yeung WSB. Adrenomedullin enhances the progressive motility and zona pellucida-binding capacity of spermatozoa from normozoospermic but not asthenozoospermic men. Molecular Reproduction and Development 2012;79(2): 76. [PubMed]

  • Lam KK, Pang RT, Lee CL, Koistinen H, Seppala M, Ho PC, Yeung WS, Chiu PC. Glycodelin-A modulates syncytialization of human BeWo choriocarcinoma cell line. Placenta. 2012 Sep;33(9):750-2 [PubMed]

  • Lee CL, Lam EY, Lam KK, Koistinen H, Seppälä M, Ng EH, Yeung WS, Chiu PC. Glycodelin-A stimulates interleukin-6 secretion by human monocytes and macrophages through L-selectin and the extracellular signal-regulated kinase pathway. J Biol Chem. 2012 Oct 26;287(44):36999-7009. [PubMed]

  • So KH, Lee CL, Yeung WS, Lee KF. Glycodelin suppresses endometrial cell migration and invasion but stimulates spheroid attachment. Reprod Biomed Online. 2012;24(6):639-45. [PubMed]

  • Lam KK, Chiu PC, Lee CL, Pang RT, Leung CO, Koistinen H, Seppala M, Ho PC, Yeung WS. Glycodelin-A protein interacts with Siglec-6 protein to suppress trophoblast invasiveness by down-regulating extracellular signal-regulated kinase (ERK)/c-Jun signaling pathway. J Biol Chem. 2011 Oct 28;286(43):37118-27. [PubMed]

  • Lee CL, Chiu PC, Lam KK, Siu SO, Chu IK, Koistinen R, Koistinen H, Seppälä M, Lee KF, Yeung WS. Differential actions of glycodelin-A on Th-1 and Th-2 cells: a paracrine mechanism that could produce the Th-2 dominant environment during pregnancy. Hum Reprod. 2011 Mar;26(3):517-26. [Medline]

  • Lee CL, Chiu PC, Pang PC, Chu IK, Lee KF, Koistinen R, Koistinen H, Seppälä M, Morris HR, Tissot B, Panico M, Dell A, Yeung WS. Glycosylation failure extends to glycoproteins in gestational diabetes mellitus: evidence from reduced α2-6 sialylation and impaired immunomodulatory activities of pregnancy-related glycodelin-A. Diabetes. 2011 Mar;60(3):909-17. [Medline]

  • Lee CL, Lam KK, Koistinen H, Seppala M, Kurpisz M, Fernandez N, Pang RT, Yeung WS, Chiu PC. Glycodelin-A as a paracrine regulator in early pregnancy. J Reprod Immunol. 2011 Jun;90(1):29-34.

  • Pang PC, Chiu PC, Lee CL, Chang LY, Panico M, Morris HR, Haslam SM, Khoo KH, Clark GF, Yeung WS, Dell A. Human sperm binding is mediated by the sialyl-Lewis(x) oligosaccharide on the zona pellucida. Science. 2011 Sep 23;333(6050):1761-4. [PubMed]

  • Chiu PC, Wong BS, Lee CL, Lam KK, Chung MK, Lee KF, Koistinen R, Koistinen H, Gupta SK, Seppälä M, Yeung WS. Zona pellucida-induced acrosome reaction in human spermatozoa is potentiated by glycodelin-A via down-regulation of extracellular signal-regulated kinases and up-regulation of zona pellucida-induced calcium influx. Hum Reprod 2010;25:2721-33.

  • Lee CL, Chiu CN, Lam KW, Chan RWS, Chu IK, Koistinen R, Koistinen H, Seppala M, Lee KF, Yeung WSB. Glycodelin-A modulates cytokine production of peripheral blood natural killer cells. Fertil Steril 2010;94:769-71. (Publication No. : 177299) 

  • Lee CL, Jiang P, Sit WH, Yang X, Wan JMF. Regulatory properties of polysaccharopeptide derived from Coriolus versicolor and its combined effect with ciclosporin on the homeostasis of human lymphocytes., Journal of Pharmacy and Pharmacology 2010;62:1028-36. (Publication No. : 180040) 

  • Lee CL, Pang PC, Yeung WS, Tissot B, Panico M, Lao TT, Chu IK, Lee KF, Chung MK, Lam KK, Koistinen R, Koistinen H, Seppälä M, Morris HR, Dell A, Chiu PC. Effects of differential glycosylation of glycodelins on lymphocyte survival. J Biol Chem. 2009 May 29;284(22):15084-96. [PubMed]

  • Chiu PC, Wong BS, Lee CL, Pang RT, Lee KF, Sumitro SB, Gupta SK, Yeung WS. Native human zona pellucida glycoproteins: purification and binding properties. Hum Reprod. 2008 Jun;23(6):1385-93. [PubMed]

Research Grants

  • Ng EHY, Chiu PCN, Lee CL, Yeung WSB. Control of human decidual macrophage differentiation by glycodelin-A. University Grants Committee of Hong Kong (UGC) Research Grants Council (RGC) General research Fund. HK$1,297,160 (2012-2014)
  • Lee CL, Chiu PCN, Yeung WSB. Differential effects of glycodelin-A on human T-helper cell subsets: A mechanism producing an immune privilege environment for fetal development. The University of Hong Kong. Small Project Funding. HK$56,457 (2010-2012).

Awards and Honors

  • Strategic Research Areas and Themes (Development and Reproduction) Best Research Output Award - 2nd Prize. Lee CL, Chiu PCN, Pang PC, Chu IK, Lee KF, Koistinen R, Koistinen H, Seppälä M, Morris HR, Tissot B, Panico M, Dell A and Yeung WSB. Glycosylation failure extends to glycoproteins in gestational diabetes: Evidence from reduced alpha 2-6 sialylation and impaired immunomodulatory activities of pregnancy-related glycodelin-A: Centre for Reproduction, Development and Growth, The University of Hong Kong